Who is spallanzani
After a few days, Needham observed that the broth had become cloudy and a single drop contained numerous microscopic creatures. He argued that the new microbes must have arisen spontaneously. In reality, however, he likely did not boil the broth enough to kill all preexisting microbes.
This suggested that microbes were introduced into these flasks from the air. Any subsequent sealing of the flasks then prevented new life force from entering and causing spontaneous generation Figure 2. Figure 2. The debate over spontaneous generation continued well into the nineteenth century, with scientists serving as proponents of both sides. To settle the debate, the Paris Academy of Sciences offered a prize for resolution of the problem.
Louis Pasteur, a prominent French chemist who had been studying microbial fermentation and the causes of wine spoilage, accepted the challenge. If a life force besides the airborne microorganisms were responsible for microbial growth within the sterilized flasks, it would have access to the broth, whereas the microorganisms would not. He correctly predicted that sterilized broth in his swan-neck flasks would remain sterile as long as the swan necks remained intact.
However, should the necks be broken, microorganisms would be introduced, contaminating the flasks and allowing microbial growth within the broth. Never will the doctrine of spontaneous generation recover from the mortal blow of this simple experiment. Figure 3. In the first part, the broth in the flask was boiled to sterilize it. When this broth was cooled, it remained free of contamination. In the second part of the experiment, the flask was boiled and then the neck was broken off.
The broth in this flask became contaminated. Which of the following individuals is credited for definitively refuting the theory of spontaneous generation using broth in swan-neck flask? Which of the following experimented with raw meat, maggots, and flies in an attempt to disprove the theory of spontaneous generation.
Skip to main content. The Cell. As a result of these studies he gave experimental proof of the action of gastric juice on foodstuffs. He theorized that this action was not putrefaction or vinous fermentation, as others had thought, but acid fermentation; however, he was unable to isolate acid from the gastric mixture.
His experiments on respiration provided evidence that tissues use oxygen and release carbon dioxide. Especially noteworthy is the long trip Spallanzani undertook in Sicily and the neighboring volcanic areas.
With systematic measurements and exact physical methods he established that there was nothing mysterious about the fire in the volcanoes; on the contrary, the same physical laws which apply on the surface of the earth are the ones which create volcanoes and which are acting in the heart of the earth.
He succeeded in measuring the heat in one of the volcanoes and expressing it in degrees Fahrenheit, which were used in regular terrestrial measuring. His description of these areas is rich—not only in describing nature but also the social habits, customs, and crafts of the inhabitants and the ways of primitive science. All rights reserved.
While at Bologna he developed an interest in natural history, which was probably encouraged by his cousin, Laura Bassi, who was professor of physics there. After receiving his doctorate he took minor orders and a few years later became a priest, although he continued to pursue his researches into natural history.
He took solutions in which microorganisms normally breed and boiled them for 30 to 45 minutes before placing them in sealed flasks. No microorganisms developed, demonstrating that Needham's broth had not been boiled for long enough to sterilize it. Opponents of Spallanzani asserted, however, that he had destroyed a vital principle in the air by prolonged boiling. While conducting these experiments, Spallanzani showed that some organisms can survive for long periods in a vacuum: this was the first practical demonstration of anaerobiosis the ability to live and grow without free oxygen.
In he submitted papers to the Royal Society on his findings concerning the regeneration of amputated parts in lower animals, and on the strength of this was elected a fellow of the Royal Society. In the same year Maria Theresa of Austria appointed Spallanzani to the chair of natural history at Pavia, which at that time was under Austrian dominion, and here he remained until his death.
He was also in charge of the museum at Pavia and made many journeys around the Mediterranean collecting natural-history specimens for the museum.
Spallanzani's research interests covered a wide area and during his career he made important contributions to the understanding of digestion, reproduction, respiration, and blood circulation, as well as sensory perception in bats. He also in managed to accomplish the artificial insemination of a dog.
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